HIstology and cytology are integral components of cell biology. a fundamental understanding of  how to carry out a histological or cytochemical experiment is necessary to appreciate the relative strengths and weakness  of these techniques. Imagine that you are working as a researcher in a cell biology lab in which yor are interested in mapping the asymetric distribution of the plasma membrane Calcium ATPas(PMCA) and sodium, calcium exchanger(NCX) to different regions of the oplasma membrane of neurons from brain tissue slices.your advisor ask  U to design a microscopy based experiment in which you must simultaneously show the localization of these enzymes. Throughly design an experiment explaining the techniques, reagents, necessary coontrols, quantitation and statistical treatments you would need to carry out this experiment.

Any idea??????