Felicity I have used the software for ELISA.However, I have no idea what you are talking about. I used the software just to get the absorbance reading, not to draw the curve. The reason is, I always have my samples in triplicate. Some of the samples falls way off from the rest of the sample. In that case I do not want them to screw up my data. So I just copy the data into excel, throw away the weird points and find mean and standard deviatin using excel. I have seen a lot of people doing the standard curve using the software too, thats also fine as long as the data is not too far from each other. The best way to figure out the trouble is to read the manual that comes with the machine.