Posted by: elusivecat November 19, 2008
Any Micr-biologists??
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We r in the eve of one more semester and like always we got project,paper,unknown dues. I m working on my bacterial unknown for my micro-bio class.I think I figured out the unknowns but not quite sure.So,I tried to do the confirmatory test for each of those bacterias I got.I did research on the internet but all I could find was ELISA test,cytometry test,DNA sequencing test.These tests are too much complex which requires sophisticated equipments which I don't have access to.So,I would appreciate if sb looks at mah result and suggest me a confirmatory test.

  MY RESULT:

 Streptococcus salivarius

Gram stain test:  +ve  Strept. Cocci

Catalase test:  -ve No bubbles formed when hydrogen peroxide was added.

Gas  from glucose/dextrose:  +ve (in this test we inoculated bacteria in a test tube containing   minitube which had air on top but after few days the color of the solution was changed to yellow and the airlayer was displaced. )

Growth on EMB agar (acid from mannitol test): -ve

Bacillus cereus:

Gram test: +ve bacilli

Catalase test: +ve

Endospore test: +ve

Growth on EMB agar: -ve

Lactobacillus fermentum

Gram stain test: +ve bacilli

Catalase test: -ve

Gas from glucose/dextrose test: +ve

 

 

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