lid 85deg ; Wait; Auto : preheats the PCR device to 85 deg and hold the temp at 85
95deg 5min temp goes up to 95 and stays there for 5 min
95deg 30 sec Cycle begins. First step is to heat the sample upto 95 deg and hold it for 30 sec
40deg 60 sec Second step of the cycle. Temp is lowered to 40 deg and holds at 40 for 60 sec
72deg 30 sec THird step. Temp goes up to 72 and hols the sample for 30 sec at 72. 1 Cycle ends.
Goto 2 rep 40 times Repeats the above cycle for 40 times
72deg 5 minutes After 40 repeats brings the sample to 72 deg and holds the sample at 72 deg for 5 min.End of PCR.
Hold 4 deg Stores your sample at 4Deg.
Hope it helps.
Sparrow
Posted by: jacksparrow September 28, 2011
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I think following happens.